The huARdb database is now updating to the v2 version and under maintanence for a while. Please contact ziwei.21@intl.zju.edu.cn for requesting datasets.

Database Aims

T-cell receptors (TCRs) and B-cell receptors (BCRs) are critical in recognizing antigens and activating the adaptive immune response. Stochastic V(D)J recombination generates massive TCR/BCR repertoire diversity. Single-cell immune profiling with transcriptome analysis allows the high-throughput study of individual TCR/BCR clonotypes and functions under both normal and pathological settings. However, a comprehensive database linking these data is not yet readily available. Here, we present the human Antigen Receptor database (huARdb), a large-scale human single-cell immune profiling database that contains 444 794 high confidence T or B cells (hcT/B cells) with full-length TCR/BCR sequence and transcriptomes from 215 datasets. We also developed a multi-functional and user-friendly web interface that provides interactive visualization modules for biologists to analyze the transcriptome and TCR/BCR features at the single-cell level.

huARdb v1 has been published on Nucleic Acid Research.

Please cite : Wu et al. (2021) huARdb: human Antigen Receptor database for interactive clonotype-transcriptome analysis at the single-cell level, Nucleic Acids Research. https://doi.org/10.1093/nar/gkab857

Figure 1 from Wu et al. (2021)

Browse and Visualize Datasets

Browse datasets by sample ID, tissue sources, cell sources, and disease types.

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	Sample ID	Receptor Type	Tissue	Tissue Metatype	Cell Subsets	Disease	Disease Metatype	Disease Status	Treatment	Donor Sex	Donor Age	A_1	A_2	B_1	B_2	C_1	C_2	DPB1_1	DPB1_2	DQA1_1	DQA1_2	DQB1_1	DQB1_2	DRB1_1	DRB1_2	Cell Number	Gene Counts	Author	DOI	Accession for Project	Accession for GEX	Accessionfor TCR	Accession for BCR

Understand the mystery of antigen receptors

Both B cells and T cells serve a crucial role in the adaptive immune response to antigens. Antibodies secreted by plasma B cells can bind to specific proteins from bacteria and viruses and neutralize these pathogenic antigens. The heavy and light chains of the antibodies, which also compose the B cell receptors (BCRs), have a critical role in recognizing the epitope of the antigens. The antibodies and BCRs are highly diverse, resulting in almost infinite diversity to recognize a great variety of antigens

Like B cells, T cells are produced in the bone marrow, and their surface displays antigen receptors called T cell receptors (TCRs). In the T-cell mediated adaptive immune response, protein antigens are presented to TCRs by major histocompatibility complex (MHC) by antigen-presenting cells (APCs). The binding between TCRs and antigens activates T cells and triggers T cell response.

Building single-cell immune profiling repository

The increasing amount of single-cell immune profiling data provides an additional and important layer of information to the understanding of the adaptive immune response, while it also brings challenges for analyzing and integrating datasets generated from different patients, and studies. Batch effects as results of variations in sequencing depth, sample preparation, and heterogeneity in tumour microenvironment and patient disease settings often confound the interpretation of the dataset. To provide a comprehensive resource of single-cell immune profiling data, we have previously described huARdb, which exploits single-cell immune profiling and transcriptomics to reveal individual clonotypes of TCRs and BCRs (Wu et al., 2021)